BS314-6-AU-CO:
Structural and Molecular Enzymology

The details
2019/20
Life Sciences (School of)
Colchester Campus
Autumn
Undergraduate: Level 6
Current
Thursday 03 October 2019
Saturday 14 December 2019
15
21 May 2019

 

Requisites for this module
BS232
(none)
(none)
(none)

 

(none)

Key module for

BSC C700 Biochemistry,
BSC C701 Biochemistry (Including Placement Year),
BSC C703 Biochemistry (Including Year Abroad),
BSC CR00 Biochemistry (Including Foundation Year)

Module description

You will already have studied simple single site enzymes and have analysed their steady state kinetic behaviour in terms of a model in which an enzyme/substrate complex forms and the substrate is transformed in the active site to yield the product. This model gives directly Michaelis Menten kinetics. This module will begin by considering the steady state kinetic mechanisms of some two-substrate enzymes. We will take a close look at the mechanism of the large class of enzymes termed dehydrogenases and how kinetic measurements and structural investigations enable plausible mechanisms to be deduced.

We also wish to examine more complex systems that comprise many sites which interact through linked protein conformational changes; Allosteric systems. Such systems lead to cooperativity in which initial binding of substrate leads to enhanced (or diminished) binding of further substrate. In this module we will examine such systems and enquire how these effects are produced and what are the biochemical and physiological benefits to the organism of both positive and negative cooperativity. The mechanisms through which allosteric effectors modulate the behaviour of allosteric proteins will be examined and their role in controlling protein action studied. Now that high-resolution structures are available we are able to understand how some of these systems function at the molecular level and a number of examples will be chosen to illustrate the molecular basis of allostery.

Module aims

This module aims to build upon knowledge gained in previous years

Module learning outcomes

To pass this module students will need to be able to:

1. discuss models (mathematical and structural) of enzyme activity, the mechanisms of allostery and the experimental basis on which the various models of allostery may be distinguished;
2. discuss the mechanism of action of the dehydrogenases and the steady state mechanisms of multi-site enzymes;
3. use key skills, particularly those related to mathematical modelling in the analysis of experimental data.

Module information

No additional information available.

Learning and teaching methods

Lectures: 18 x 1 hr including a total of 2hr data analysis and interpretation. Classes: 2 x 1 hr Student managed learning: 130 hours/module Total: 150 hours

Bibliography

This module does not appear to have any essential texts. To see non-essential items, please refer to the module's reading list.

Overall assessment

Coursework Exam
0% 100%

Reassessment

Coursework Exam
0% 100%
Module supervisor and teaching staff
Dr Brandon Reeder
School Undergraduate Office, email: bsugoffice (Non essex users should add @essex.ac.uk to create the full email address)

 

Availability
Yes
No
No

External examiner

Dr Clive Butler
The University of Exeter
Associate Professor of Microbial Biochemistry
Resources
Available via Moodle
Of 22 hours, 22 (100%) hours available to students:
0 hours not recorded due to service coverage or fault;
0 hours not recorded due to opt-out by lecturer(s).

 

Further information
Life Sciences (School of)

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